海军医学杂志

目的观察半夏总生物碱(total alkaloids from Pinellia Ternate,TATP)对人胃癌细胞株SGC-7901增殖抑制的影响,探讨其可能的作用机制。方法采用四甲基偶氮唑盐比色法及集落形成率实验,检测不同浓度的TATP对SGC-7901细胞株的生长抑制作用;应用单细胞凝胶电泳技术检测TATP导致SGC-7901细胞的DNA损伤情况。结果人胃癌细胞SGC-7901经TATP处理后,其体外增殖能力明显下降,且与药物的剂量、干预时间呈正相关,与对照组比较,差异均有统计学意义(P<0.05或<0.01)。单细胞凝胶电泳(single cell gel electrophoresis,SCGE)中,TATP组细胞尾DNA含量、尾长及尾动量与对照组比较差异均有统计学意义(P<0.01),且呈现一定的浓度依赖性。结论一定剂量的TATP能明显抑制SGC-7901细胞的增殖,其作用机制可能与细胞DNA的损伤有关。

Objective To observe the inhibitive effect of total alkaloids from Pinellia Ternate( TATP) on the proliferation on the human gastric cancer cell strain SGC-7901,and to investigate possible mechanism involved. Methods The inhibitive effect of different level TATP on the proliferation of SGC-7901 treated with TATP was monitored with the methyl thiazolil tetracolium colorimetic method and the plate clone formation assay. DNA damage of SGC-7901 cells induced by TATP was detected with the single cell gel electrophoresis( SCGE).Results Treated with TATP,the in vitro proliferation of SGC-7901 was significantly decreased,which was positively correlated with the dosage of the drug and intervention time. Statistical significance could be seen,when it was compared with that of the control group( P <0. 05).Single cell gel electrophoresis( SCGE) indicated that significant differences could be seen in cell tail DNA contents,tail length and intensity of tail movement for the TATP experimental group and were dose-dependent( P <0. 01). Conclusion A certain dosage of TATP could inhibit the proliferation of SGC-7901 in vitro,the mechanism of which might be correlated to the DNA damage induced by TATP.