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目的探讨天香胶囊对运动病(motion sickness,MS)模型大鼠行为学的影响及其机理。方法将60只SPF级雄性SD大鼠随机分为6组:空白对照组、模型对照组、阳性对照组、天香胶囊低、中、高剂量组,每组10只,各组大鼠在造模前1 h给药,采用旋转刺激装置持续刺激2 h,诱发大鼠运动病模型,采用自发活动实验观察大鼠行为学变化、ELISA和放免法检测前庭核乙酰胆碱(ACh)含量和血浆促肾上腺皮质激素(ACTH)、β-内啡肽(β-EP)水平。结果天香胶囊各剂量组均可增加运动病模型大鼠的自发活动总距离和活动时间,减低血浆ACTH[空白对照组(24.48±2.28)ng/L,天香低剂量组(30.56±4.26)ng/L]和β-EP[空白对照组(141.27±12.11)ng/L,天香低剂量组(164.03±23.60)ng/L]含量以及前庭核ACh[空白对照组(58.87±6.03)ng/L,天香低剂量组(67.78±10.34)ng/L]含量,且显示出一定的剂量依赖关系。结论天香胶囊可显著改善运动病模型大鼠的自发活动,其机制可能与缓解中枢神经系统兴奋性降低,降低血浆ACTH、β-EP含量以及前庭核ACh含量有关,提示中药复方天香胶囊对运动病有一定防治作用,其高剂量对模型大鼠作用效果显著,为最佳剂量。
Abstract：Objective To study the effect of Tianxiang capsules on the behavior of rats with motion sickness and its related mechanism. Methods A total of 60 male SD rats were randomly divided into 6 groups: the blank control group,the model group,the scopolamine group( or the positive control group),the Tianxiang low,moderate and high dosage groups,each consisting of 10 rats.Rats in each group were given medication 1 hour before development of the model,and the animals were exposed continuously to rotating stimulation for 2 hours by using a rotational stimulation device,and the motion sickness model was thus developed. Spontaneous activity was used to observe changes in the behavior of rats,the levels of acetylcholine( ACh),adrenocorticotropic hormone( ACTH) and β-endorphin( β-EP) were detected by using ELISA and radioimmunoassay. Results Total distance and duration of spontaneous activity of the rats in the 3 different Tianxiang dosage groups were all increased to various extents,and the levels of ACTH,β-EP and ACh were decreased in vestibular nucleus,and a dose-dependent relationship could clearly be seen. Conclusion The Tianxiang capsule could significantly improve the spontaneous activity of the rats with motion sickness,the intrinsic mechanism might be related to the decrease of excitability of central nervous system,and the reduction of plasma ACTH,β-EP and ACh levels of vestibular nucleus. It was indicated that the compound Tianxiang capsule had certain prevention and treatment effects on motion sickness,and high dosage could produce most significant effects on the model rats,and it was obviously the optimal dosage.
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基本信息:
中图分类号:R285.5;R-332
引用信息:
[1]张炜悦,李卫红,胡艳红,等.天香胶囊对运动病模型大鼠行为学的影响及其机制[J].海军医学杂志,2018,39(01):25-28.
基金信息:
国家中医药管理局重点学科北京中医药大学航海中医药学重点学科(No.S22/0100604054)
2018-01-28
2018-01-28